Moleculer Cytogenetics (FISH)

List of Molecular Cytogenetics (FISH) Tests

S.No Test name Sample Type Sample Volume Vacutainer TAT*
1 FISH for any one or combination 9,13,14,15,16,18,21,22 Peripheral blood / Amniotic FluidCVS / Tissue 3ml / 5-10ml Green top Sodium Heparin / Sterile Tube / Sterile Container / Normal Saline 05 Days
2 FISH for any one or combination 9,13,14,15,16,18,21,22,X Y Peripheral bloodProducts of Conception / Abortion Material 3ml Green top Sodium Heparin / Sterile Tube 5 Days
3 Spectral karyotyping or chromosome painting 24 color Peripheral blood / Amniotic Fluid 3ml / 5-10ml Green top Sodium Heparin / Sterile Tube 05 Days
4 Pre-implantation Genetic Diagnosis-PGD Single cell from embryo on Fixed slide Call

FISH - Microdeletions

S.No Test name Sample Type Sample Volume Vacutainer TAT*
1 Digeorge Syndrome (VCFS region) Peripheral blood / Amniotic Fluid 3ml / 10ml Green Top Na Heparin / Sterile Tube 5 Days
2 Digeorge Syndrome (TBX1 probe) Peripheral blood / Amniotic Fluid 3ml / 10ml Green Top Na Heparin / Sterile Tube 5 Days
3 Miller-Decker Syndrome Peripheral blood / Amniotic Fluid 3ml / 10ml Green Top Na Heparin / Sterile Tube 5 Days
4 Prader-Willi Syndrome Peripheral blood / Amniotic Fluid 3ml / 10ml Green Top Na Heparin / Sterile Tube 5 Days
5 Smith-Magenis Syndrome Peripheral blood / Amniotic Fluid 3ml / 10ml Green Top Na Heparin / Sterile Tube 5 Days
6 Williams Syndrome Peripheral blood / Amniotic Fluid 3ml / 10ml Green Top Na Heparin / Sterile Tube 5 Days
7 Wolf Hirschhorn Syndrome 4p deletion (TelVysion 4p probe) Peripheral blood / Amniotic Fluid 3ml / 10ml Green Top Na Heparin / Sterile Tube 5 Days
8 1p36 deletion syndrome Peripheral blood / Amniotic Fluid 3ml / 10ml Green Top Na Heparin / Sterile Tube 5 Days
9 Other Microdeletions Peripheral blood / Amniotic Fluid 3ml / 10ml Green Top Na Heparin / Sterile Tube Call

FISH - Cancer Mutations

S.No Test name Sample Type Sample Volume Vacutainer TAT*
1 bcr/abl (CML) FISH Peripheral blood 3ml Green Top Na Heparin 5 Days
2 EGFR mutation (Lung small cell cancer) Peripheral blood 3ml Green Top Na Heparin 5 Days
3 Her 2 gene amplification (Breast Cancer) Peripheral blood 3ml Green Top Na Heparin 5 Days
4 Translocation 15/17 (PML/RARa for APL) Peripheral blood 3ml Green Top Na Heparin 5 Days
5 Translocation 8/14 (Burkitts, Lymphoma) Peripheral blood 3ml Green Top Na Heparin 5 Days
6 Translocation 8/21 (AML) Peripheral blood 3ml Green Top Na Heparin 5 Days
7 Retinoblastoma Peripheral blood 3ml Green Top Na Heparin 5 Days

Prenatal COMBO Cyto / Fish Tests

S.No Test name Sample Type Sample Volume Vacutainer TAT*
1 Amniotic Fluid karyotyping + FISH for any aneuploidy of autosomes Amniotic Fluid 20 ml Sterile tube 2-3 wks
2 Chorionic Villus karyotyping + FISH for any aneuploidy of autosomes Chorionic Villus 5-10 mg Sterile container normal Saline 2-3 wks

 

*TAT = Maximum turn around time